Finally, after analyzing the GC ratio (%), free energy of folding-binding, melting temperature and efficacy prediction of all the predicted siRNAs, 6 siRNA molecules were selected which are proposed to exert the highest action. A total of 38 siRNAs were predicted that can inactivate RdRp and 3C-like proteinase genes. 12 conserved sequences were obtained from the genome 101 SARS-CoV-2 strains sequenced in different countries. In the current study, siRNA molecules were designed against RdRp and 3C-like proteinase gene. Among various treatment options, siRNA, also known as small interfering RNA is a prospective tool of the RNA interference (RNAi) pathway that controls virus-mediated infections of human by suppressing the expression of viral gene through hybridization and neutralization of complementary mRNA. Two proteins, namely, RNA-dependent RNA polymerase (RdRp) and 3C-like proteinase work in facilitating the entry of the virus into host cell, viral transcription and replication. This has led to the race of scientists all over the world to discover a therapeutic to combat the SARS-CoV-2. Since the origin of the SARS-CoV2 from Wuhan, in China, it has become a global threat to public health and a WHO-declared world epidemic within 2 month of identification of the first case.
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